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KD-Validated Anti-Homeobox B4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Homeobox B4 Rabbit Monoclonal Antibody AGI1038
    Western blotting analysis using anti-homeobox B4 antibody (Cat#AGI1038). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-homeobox B4 antibody (Cat#AGI1038, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Homeobox B4 Rabbit Monoclonal Antibody AGI1038
    Western blotting analysis using anti-homeobox B4 antibody (Cat#AGI1038). Homeobox B4 expression in wild type (WT) and homeobox B4 (HOXB4) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-homeobox B4 antibody (Cat#AGI1038, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Homeobox B4 Rabbit Monoclonal Antibody AGI1038
    Flow cytometric analysis of homeobox B4 expression in HAP-1 cells using anti-homeobox B4 antibody (Cat#AGI1038, 1:2,000). Green, isotype control; red, homeobox B4.
    detail
  • ICC - KD-Validated Anti-Homeobox B4 Rabbit Monoclonal Antibody AGI1038
    Immunocytochemical staining of HAP-1 cells with anti-Homeobox B4 antibody (Cat#AGI1038, 1:1,000). Nuclei were stained blue with DAPI; Homeobox B4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P17483
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB6010
Calculated MW Predicted, 28 kDa, observed, 34 kDa
Gene Name HOXB4
Aliases HOXB4; Homeobox B4; HOX2F; Homeobox Protein Hox-2.6; Homeobox Protein Hox-B4; Homeobox Protein Hox-2F; Homeo Box B4; HOX2; Homeo Box 2F; HOX-2.6
Immunogen A synthesized peptide derived from human HOXB4
Additional Information
Gene ID 3214
Other Names Homeobox protein Hox-B4, Homeobox protein Hox-2.6, Homeobox protein Hox-2F, HOXB4, HOX2F
Protein Information
Name HOXB4
Synonyms HOX2F
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Cellular Location Nucleus.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1038
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