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KD-Validated Anti-GTF2F2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-GTF2F2 Rabbit Monoclonal Antibody AGI1045
    Western blotting analysis using anti-GTF2F2 antibody (Cat#AGI1045). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GTF2F2 antibody (Cat#AGI1045, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-GTF2F2 Rabbit Monoclonal Antibody AGI1045
    Western blotting analysis using anti-GTF2F2 antibody (Cat#AGI1045). GTF2F2 expression in wild type (WT) and GTF2F2 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-GTF2F2 antibody (Cat#AGI1045, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • ICC - KD-Validated Anti-GTF2F2 Rabbit Monoclonal Antibody AGI1045
    Immunocytochemical staining of HepG2 cells with anti-GTF2F2 antibody (Cat#AGI1045, 1:1,000). Nuclei were stained blue with DAPI; GTF2F2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P13984
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB6035
Calculated MW Predicted, 28 kDa, observed, 30 kDa
Gene Name GTF2F2
Aliases GTF2F2; General Transcription Factor IIF Subunit 2; RAP30; TFIIF; BTF4; General Transcription Factor IIF, Polypeptide 2, 30kDa; Transcription Initiation Factor IIF Subunit Beta; General Transcription Factor IIF 30 KDa Subunit; Transcription Initiation Factor RAP30; TFIIF-Beta; General Transcription Factor IIF, Polypeptide 1 (74kD Subunit); ATP-Dependent Helicase GTF2F2; EC 3.6.1; TF2F2
Immunogen A synthesized peptide derived from human GTF2F2
Additional Information
Gene ID 2963
Other Names General transcription factor IIF subunit 2, General transcription factor IIF 30 kDa subunit, Transcription initiation factor IIF subunit beta, TFIIF-beta, Transcription initiation factor RAP30, GTF2F2, RAP30
Protein Information
Name GTF2F2
Synonyms RAP30
Function TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.
Cellular Location Nucleus.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1045
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