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KD-Validated Anti-PSMB10 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PSMB10 Rabbit Monoclonal Antibody AGI1075
    Western blotting analysis using anti-PSMB10 antibody (Cat#61197). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMB10 antibody (Cat#61197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-PSMB10 Rabbit Monoclonal Antibody AGI1075
    Western blotting analysis using anti-PSMB10 antibody (Cat#61197). PSMB10 expression in wild type (WT) and PSMB10 shRNA knockdown (KD) Hela cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PSMB10 antibody (Cat#61197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-PSMB10 Rabbit Monoclonal Antibody AGI1075
    Flow cytometric analysis of PSMB10 expression in H9c2 cells using PSMB10 antibody (Cat#61197, 1:2,000). Green, isotype control; red, PSMB10.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P40306
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2165
Calculated MW Predicted, 29 kDa, observed, 27 kDa
Gene Name PSMB10
Aliases PSMB10; Proteasome 20S Subunit Beta 10; LMP10; MECL1; Proteasome (Prosome, Macropain) Subunit, Beta Type, 10; Multicatalytic Endopeptidase Complex Subunit MECl-1; Proteasome Subunit Beta Type-10; Low Molecular Mass Protein 10; Proteasome Subunit Beta 10; Proteasome Subunit Beta-2i; Macropain Subunit MECl-1; Proteasome MECl-1; EC 3.4.25.1; MGC1665; Beta2i; Proteasome Catalytic Subunit 2i; Proteasome Subunit Beta 7i; Proteasome Subunit Beta2i; Proteasome Subunit MECL1; Proteasome Subunit Β2i; PRAAS5; BETA2I
Immunogen A synthesized peptide derived from human PSMB10
Additional Information
Gene ID 5699
Other Names Proteasome subunit beta type-10, 3.4.25.1, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i, PSMB10, LMP10, MECL1
Protein Information
Name PSMB10
Synonyms LMP10, MECL1
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
Cellular Location Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU00809}. Nucleus
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1075
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