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KD-Validated Anti-Pancreatic proteinase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Pancreatic proteinase 1 Rabbit Monoclonal Antibody AGI1080
    Western blotting analysis using anti-pancreatic proteinase 1 antibody (Cat#AGI1080). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pancreatic proteinase 1 antibody (Cat#AGI1080, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Pancreatic proteinase 1 Rabbit Monoclonal Antibody AGI1080
    Western blotting analysis using anti-pancreatic proteinase 1 antibody (Cat#AGI1080). Pancreatic proteinase 1 expression in wild-type (WT) and pancreatic proteinase 1 (HPRT1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-pancreatic proteinase 1 antibody (Cat#AGI1080, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Pancreatic proteinase 1 Rabbit Monoclonal Antibody AGI1080
    Flow cytometric analysis of pancreatic proteinase 1 expression in HepG2 cells using pancreatic proteinase 1 antibody (Cat#AGI1080, 1:2,000). Green, isotype control; red, pancreatic proteinase 1.
    detail
  • ICC - KD-Validated Anti-Pancreatic proteinase 1 Rabbit Monoclonal Antibody AGI1080
    Immunocytochemical staining of HepG2 cells with anti-pancreatic proteinase 1 antibody (Cat#AGI1080, 1:1,000). Nuclei were stained blue with DAPI; Pancreatic proteinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P00492
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB6185
Calculated MW Predicted, 25 kDa, observed, 25 kDa
Gene Name HPRT1
Aliases HPRT1; Hypoxanthine Phosphoribosyltransferase 1; HGPRT; HPRT; Hypoxanthine Guanine Phosphoribosyl Transferase; Hypoxanthine-Guanine Phosphoribosyltransferase; EC 2.4.2.8; HGPRTase; Hypoxanthine-Guanine Phosphoribosyltransferase 1; Testicular Tissue Protein Li 89; Lesch-Nyhan Syndrome
Immunogen A synthesized peptide derived from human HPRT
Additional Information
Gene ID 3251
Other Names Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, 2.4.2.8, HPRT1, HPRT
Protein Information
Name HPRT1
Synonyms HPRT
Function Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5- phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1080
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