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KD-Validated Anti-Transcription elongation factor A1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Transcription elongation factor A1 Rabbit Monoclonal Antibody AGI1101
    Western blotting analysis using anti-Transcription elongation factor A1 antibody (Cat#AGI1101). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Transcription elongation factor A1 antibody (Cat#AGI1101, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Transcription elongation factor A1 Rabbit Monoclonal Antibody AGI1101
    Western blotting analysis using anti-Transcription elongation factor A1 antibody (Cat#AGI1101). Transcription elongation factor A1 expression in wild type (WT) and transcription elongation factor A1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Transcription elongation factor A1 antibody (Cat#AGI1101, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Transcription elongation factor A1 Rabbit Monoclonal Antibody AGI1101
    Flow cytometric analysis of Transcription elongation factor A1 expression in HepG2 cells using Transcription elongation factor A1 antibody (Cat#AGI1101, 1:2,000). Green, isotype control; red, Transcription elongation factor A1.
    detail
  • ICC - KD-Validated Anti-Transcription elongation factor A1 Rabbit Monoclonal Antibody AGI1101
    Immunocytochemical staining of HepG2 cells with Transcription elongation factor A1 antibody (Cat#AGI1101, 1:1,000). Nuclei were stained blue with DAPI; Transcription elongation factor A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P23193
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2335
Calculated MW Predicted, 34 kDa, observed, 34 kDa
Gene Name TCEA1
Aliases TCEA1; Transcription Elongation Factor A1; TFIIS; GTF2S; TF2S; SII; Transcription Elongation Factor S-II Protein 1; Transcription Elongation Factor A Protein 1; Transcription Elongation Factor A (SII), 1; Transcription Elongation Factor TFIIS.O; Transcription Factor IIS; TCEA; Epididymis Secretory Sperm Binding Protein
Immunogen A synthesized peptide derived from human TCEA1
Additional Information
Gene ID 6917
Other Names Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIIS.o, TCEA1, GTF2S, TFIIS
Protein Information
Name TCEA1
Synonyms GTF2S, TFIIS
Function Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Cellular Location Nucleus.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1101
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