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KD-Validated Anti-Adenylosuccinate Lyase Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-Adenylosuccinate Lyase Mouse Monoclonal Antibody AGI1110
    Western blotting analysis using anti-adenylosuccinate lyase antibody (Cat#AGI1110). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adenylosuccinate lyase antibody (Cat#AGI1110, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Adenylosuccinate Lyase Mouse Monoclonal Antibody AGI1110
    Western blotting analysis using anti-adenylosuccinate lyase antibody (Cat#AGI1110). Adenylosuccinate lyase expression in wild type (WT) and adenylosuccinate lyase (ADSL) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adenylosuccinate lyase antibody (Cat#AGI1110, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Adenylosuccinate Lyase Mouse Monoclonal Antibody AGI1110
    Flow cytometric analysis of Adenylosuccinate lyase expression in HeLa cells using anti-Adenylosuccinate lyase antibody (Cat# AGI1110, 1:2,000). Green, isotype control; red, Adenylosuccinate lyase.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P30566
Reactivity Rat, Human
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 25GB595
Calculated MW Predicted, 55 kDa, observed, 48 kDa
Gene Name ADSL
Aliases ADSL; Adenylosuccinate Lyase; Adenylosuccinase; EC 4.3.2.2; AMPS; ASL; EC 4.3.2; ASASE; ASase
Immunogen Recombinant protein of human ADSL
Additional Information
Gene ID 158
Other Names Adenylosuccinate lyase, ADSL, ASL, 4.3.2.2, Adenylosuccinase, ASase, ADSL, AMPS
Protein Information
Name ADSL
Synonyms AMPS
Function Catalyzes two non-sequential steps in de novo AMP synthesis: converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate.
Tissue Location Ubiquitously expressed. Both isoforms are produced by all tissues. Isoform 2 is 10-fold less abundant than isoform 1
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1110
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