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KD-Validated Anti-CD99 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CD99 Rabbit Monoclonal Antibody AGI1188
    Western blotting analysis using anti-CD99 antibody (Cat#AGI1188). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD99 antibody (Cat#AGI1188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-CD99 Rabbit Monoclonal Antibody AGI1188
    Western blotting analysis using anti-CD99 antibody (Cat#AGI1188). CD99 expression in wild type (WT) and CD99 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CD99 antibody (Cat#AGI1188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-CD99 Rabbit Monoclonal Antibody AGI1188
    Flow cytometric analysis of CD99 expression in HepG2 cells using CD99 antibody (Cat#AGI1188, 1:2,000). Green, isotype control; red, CD99.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P14209
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3395
Calculated MW Predicted, 19 kDa , observed, 28 kDa
Gene Name CD99
Aliases CD99; CD99 Molecule (Xg Blood Group); CD99 Antigen; MIC2; Antigen Identified By Monoclonal Antibodies 12E7, F21 And O13; T-Cell Surface Glycoprotein E2; E2 Antigen; MIC2X; MIC2Y; Antigen Identified By Monoclonal 12E7, Y Homolog; MIC2 (Monoclonal Antibody 12E7); Cell Surface Antigen HBA-71; Cell Surface Antigen 12E7; Cell Surface Antigen O13; Surface Antigen MIC2; CD99 Molecule; Protein MIC2; HBA71; MSK5X; 12E7
Immunogen A synthesized peptide derived from human CD99
Additional Information
Gene ID 4267
Other Names CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD99, CD99, MIC2, MIC2X, MIC2Y
Protein Information
Name CD99
Synonyms MIC2, MIC2X, MIC2Y
Function Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1188
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