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KD-Validated Anti-CD97 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CD97 Rabbit Monoclonal Antibody AGI1198
    Western blotting analysis using anti-CD97 antibody (Cat#61404). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD97 antibody (Cat#61404, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-CD97 Rabbit Monoclonal Antibody AGI1198
    Western blotting analysis using anti-CD97 antibody (Cat#61404). CD97 expression in wild type (WT) and CD97 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CD97 antibody (Cat#61404, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-CD97 Rabbit Monoclonal Antibody AGI1198
    Flow cytometric analysis of CD97 expression in HeLa cells using CD97 antibody (Cat#61404, 1:2,000). Green, isotype control; red, CD97.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P48960
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3390
Calculated MW Predicted, 92 kDa, observed, 74 kDa
Gene Name ADGRE5
Aliases ADGRE5; Adhesion G Protein-Coupled Receptor E5; Leukocyte Antigen CD97; TM7LN1; CD97; Seven-Transmembrane, Heterodimeric Receptor Associated With Inflammation; Seven Transmembrane Helix Receptor; Seven-Span Transmembrane Protein; CD97 Molecule; CD97 Antigen
Immunogen A synthesized peptide derived from human CD97
Additional Information
Gene ID 976
Other Names Adhesion G protein-coupled receptor E5 {ECO:0000312|HGNC:HGNC:1711}, Leukocyte antigen CD97, CD97, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta, ADGRE5 (HGNC:1711)
Protein Information
Name ADGRE5 (HGNC:1711)
Function Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q9Z0M6}; Multi-pass membrane protein
Tissue Location Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompanied by detectable levels of soluble CD97 in the synovial fluid
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1198
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