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KD-Validated Anti-Casein kinase 2 beta Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Casein kinase 2 beta Rabbit Monoclonal Antibody AGI1219
    Western blotting analysis using anti-Casein kinase 2 beta antibody (Cat#61434). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Casein kinase 2 beta antibody (Cat#61434, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Casein kinase 2 beta Rabbit Monoclonal Antibody AGI1219
    Western blotting analysis using anti-Casein kinase 2 beta antibody (Cat#61434). Casein kinase 2 beta expression in wild type (WT) and Casein kinase 2 beta shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Casein kinase 2 beta antibody (Cat#61434, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • ICC - KD-Validated Anti-Casein kinase 2 beta Rabbit Monoclonal Antibody AGI1219
    Immunocytochemical staining of HepG2 cells with Casein kinase 2 beta antibody (Cat#61434, 1:1,000). Nuclei were stained blue with DAPI; Casein kinase 2 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P67870
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3730
Calculated MW Predicted, 25 kDa , observed, 25 kDa
Gene Name CSNK2B
Aliases CSNK2B; Casein Kinase 2 Beta; Casein Kinase 2, Beta Polypeptide; Casein Kinase II Subunit Beta; Protein G5a; CK II Beta; Phosvitin; Ckb1; Ckb2; CK2N; G5A; Alternative Name: G5a, Phosvitin; Casein Kinase II Beta Subunit; CSNK2B-LY6G5B-1072; CSNK2B-LY6G5B-1103; CSNK2B-LY6G5B-532; CSNK2B-LY6G5B-560; CSNK2B-LY6G5B-562; POBINDS; CSK2B; CK2B; CKB1; CKB2
Immunogen A synthesized peptide derived from human Casein Kinase 2 beta
Additional Information
Gene ID 1460
Other Names Casein kinase II subunit beta, CK II beta, Phosvitin, Protein G5a, CSNK2B (HGNC:2460), CK2N, G5A
Protein Information
Name CSNK2B (HGNC:2460)
Synonyms CK2N, G5A
Function Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine (PubMed:11239457, PubMed:16818610). Participates in Wnt signaling (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1219
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