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KD-Validated Anti-Calpain small subunit 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Calpain small subunit 1 Rabbit Monoclonal Antibody AGI1230
    Western blotting analysis using anti-Calpain small subunit 1 antibody (Cat#AGI1230). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Calpain small subunit 1 antibody (Cat#AGI1230, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Calpain small subunit 1 Rabbit Monoclonal Antibody AGI1230
    Western blotting analysis using anti-Calpain small subunit 1 antibody (Cat#AGI1230). Calpain small subunit 1 expression in wild type (WT) and Calpain small subunit 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Calpain small subunit 1 antibody (Cat#AGI1230, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Calpain small subunit 1 Rabbit Monoclonal Antibody AGI1230
    Flow cytometric analysis of Calpain small subunit 1 expression in HepG2 cells using Calpain small subunit 1 antibody (Cat#AGI1230, 1:2,000). Green, isotype control; red, Calpain small subunit 1.
    detail
  • ICC - KD-Validated Anti-Calpain small subunit 1 Rabbit Monoclonal Antibody AGI1230
    Immunocytochemical staining of HepG2 cells with Calpain small subunit 1 antibody (Cat#AGI1230, 1:1,000). Nuclei were stained blue with DAPI; Calpain small subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P04632
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3720
Calculated MW Predicted, 28 kDa , observed, 28 kDa
Gene Name CAPNS1
Aliases CAPNS1; Calpain Small Subunit 1; CDPS; CANPS; CAPN4; CANP; Calcium-Activated Neutral Proteinase Small Subunit; Calcium-Dependent Protease Small Subunit 1; Calpain Regulatory Subunit; CANP Small Subunit; CSS1; 30K; Epididymis Secretory Sperm Binding Protein; Calcium-Dependent Protease, Small Subunit; Calcium-Dependent Protease Small Subunit; Calpain 4, Small Subunit (30K); Calpain, Small Polypeptide; CALPAIN4; CAPNS
Immunogen A synthesized peptide derived from human Calpain small subunit 1
Additional Information
Gene ID 826
Other Names Calpain small subunit 1, CSS1, Calcium-activated neutral proteinase small subunit, CANP small subunit, Calcium-dependent protease small subunit, CDPS, Calcium-dependent protease small subunit 1, Calpain regulatory subunit, CAPNS1, CAPN4, CAPNS
Protein Information
Name CAPNS1
Synonyms CAPN4, CAPNS
Function Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development (By similarity).
Cellular Location Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon calcium binding.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1230
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