Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KD-Validated Anti-Paired box 6 Rabbit Monoclonal Antibody   

KD-Validated Anti-Paired box 6 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Paired box 6 Rabbit Monoclonal Antibody AGI1263
    Western blotting analysis using anti-Paired box 6 antibody (Cat#61495). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Paired box 6 antibody (Cat#61495, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Paired box 6 Rabbit Monoclonal Antibody AGI1263
    Western blotting analysis using anti-Paired box 6 antibody (Cat#61495). Paired box 6 expression in wild type (WT) and paired box 6 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Paired box 6 antibody (Cat#61495, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Paired box 6 Rabbit Monoclonal Antibody AGI1263
    Flow cytometric analysis of Paired box 6 expression in HeLa cells using Paired box 6 antibody (Cat#61495, 1:2,000). Green, isotype control; red, Paired box 6.
    detail
  • ICC - KD-Validated Anti-Paired box 6 Rabbit Monoclonal Antibody AGI1263
    Immunocytochemical staining of HeLa cells with Paired box 6 antibody (Cat#61495, 1:1,000). Nuclei were stained blue with DAPI; Paired box 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P26367
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2875
Calculated MW Predicted, 47 kDa , observed, 47 kDa
Gene Name PAX6
Aliases Paired Box 6; D11S812E; WAGR; AN2; AN; Paired Box Protein Pax-6; Aniridia Type II Protein; Oculorhombin; Aniridia 1; Aniridia 2; AN1; Paired Box Gene 6 (Aniridia, Keratitis); Paired Box 6 PAX6A-ASdel6-AS7 Isoform; Paired Box 6 PAX6A-ASdel6 Isoform; Paired Box 6 PAX6B-ASdel6 Isoform; Paired Box 6 PAX6A-Del6 Isoform; Paired Box 6 PAX6-12a Isoform; Paired Box 6 PAX6D Isoform; Paired Box Homeotic Gene-6; Alternative Protein PAX6; Aniridia, Keratitis; ASGD5; FVH1; MGDA;
Immunogen A synthesized peptide derived from human PAX6
Additional Information
Gene ID 5080
Other Names Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2
Protein Information
Name PAX6
Synonyms AN2
Function Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Acts as a transcriptional repressor of NFATC1- mediated gene expression (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P63015}. [Isoform 5a]: Nucleus {ECO:0000250|UniProtKB:P63016}
Tissue Location [Isoform 1]: Expressed in lymphoblasts.
Research Areas
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 149.00
$ 499.00
Cat# AGI1263
Size:
Quantity:
Availability:
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaMexicoNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"