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KD-Validated Anti-PFKM Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PFKM Rabbit Monoclonal Antibody AGI1265
    Western blotting analysis using anti-PFKM antibody (Cat#AGI1265). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PFKM antibody (Cat#AGI1265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-PFKM Rabbit Monoclonal Antibody AGI1265
    Western blotting analysis using anti-PFKM antibody (Cat#AGI1265). PFKM expression in wild type (WT) and PFKM shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PFKM antibody (Cat#AGI1265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-PFKM Rabbit Monoclonal Antibody AGI1265
    Flow cytometric analysis of PFKM expression in HepG2 cells using PFKM antibody (Cat#AGI1265, 1:2,000). Green, isotype control; red, PFKM.
    detail
  • ICC - KD-Validated Anti-PFKM Rabbit Monoclonal Antibody AGI1265
    Immunocytochemical staining of HepG2 cells with PFKM antibody (Cat#AGI1265, 1:1,000). Nuclei were stained blue with DAPI; PFKM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P08237
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB2740
Calculated MW Predicted, 85 kDa, observed, 75 kDa
Gene Name PFKM
Aliases PFKM; Phosphofructokinase, Muscle; PPP1R122; PFK-1; PFKX; ATP-Dependent 6-Phosphofructokinase, Muscle Type; Protein Phosphatase 1, Regulatory Subunit 122; Phosphofructokinase, Polypeptide X; Phosphofructo-1-Kinase Isozyme A; 6-Phosphofructokinase Type A; Phosphohexokinase; EC 2.7.1.11; ATP-PFK; PFK-A; 6-Phosphofructokinase, Muscle Type; 6-Phosphofructo-1-Kinase; Phosphofructokinase 1; Phosphofructokinase-M; EC 2.7.1; PFK-M; GSD7; PFK1; PFKA
Immunogen A synthesized peptide derived from human Fructose 6 Phosphate Kinase
Additional Information
Gene ID 5213
Other Names ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-M, 2.7.1.11 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, PFK-A, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKM, PFKX
Protein Information
Name PFKM
Synonyms PFKX
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1265
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