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KD-Validated Anti-PWP1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PWP1 Rabbit Monoclonal Antibody AGI1277
    Western blotting analysis using anti-PWP1 antibody (Cat#AGI1277). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PWP1 antibody (Cat#AGI1277, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-PWP1 Rabbit Monoclonal Antibody AGI1277
    Western blotting analysis using anti-PWP1 antibody (Cat#AGI1277). PWP1 expression in wild type (WT) and PWP1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PWP1 antibody (Cat#AGI1277, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-PWP1 Rabbit Monoclonal Antibody AGI1277
    Flow cytometric analysis of PWP1 expression in HeLa cells using PWP1 antibody (Cat#AGI1277, 1:2,000). Green, isotype control; red, PWP1.
    detail
  • ICC - KD-Validated Anti-PWP1 Rabbit Monoclonal Antibody AGI1277
    Immunocytochemical staining of Hela cells with PWP1 antibody (Cat#AGI1277, 1:1,000). Nuclei were stained blue with DAPI; PWP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q13610
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2580
Calculated MW Predicted, 56 kDa, observed, 74 kDa
Gene Name PWP1
Aliases PWP1; PWP1 Homolog, Endonuclein; IEF-SSP-9502; Periodic Tryptophan Protein 1 Homolog; Keratinocyte Protein IEF SSP 9502; Endonuclein; Nuclear Phosphoprotein Similar To S. Cerevisiae PWP1; PWP1 Homolog (S. Cerevisiae); NCLB
Immunogen A synthesized peptide derived from human PWP1
Additional Information
Gene ID 11137
Other Names Periodic tryptophan protein 1 homolog {ECO:0000312|HGNC:HGNC:17015}, Keratinocyte protein IEF SSP 9502, PWP1
Protein Information
Name PWP1
Function Chromatin-associated factor that regulates transcription (PubMed:29065309). Regulates Pol I-mediated rRNA biogenesis and, probably, Pol III-mediated transcription (PubMed:29065309). Regulates the epigenetic status of rDNA (PubMed:29065309).
Cellular Location Nucleus. Nucleus, nucleolus. Chromosome. Note=Associates with chromatin regions of rDNA.
Tissue Location High levels seen in the placenta, skeletal muscle, kidney and pancreas while lower levels were seen in the heart, brain and lung
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1277
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