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KD-Validated Anti-GFPT1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-GFPT1 Rabbit Monoclonal Antibody AGI1298
    Western blotting analysis using anti-GFPT1 antibody (Cat#AGI1298). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GFPT1 antibody (Cat#AGI1298, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-GFPT1 Rabbit Monoclonal Antibody AGI1298
    Western blotting analysis using anti-GFPT1 antibody (Cat#AGI1298). GFPT1 expression in wild type (WT) and GFPT1 knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GFPT1 antibody (Cat#AGI1298, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • ICC - KD-Validated Anti-GFPT1 Rabbit Monoclonal Antibody AGI1298
    Immunocytochemical staining of HepG2 cells with anti-GFPT1 antibody (Cat#AGI1298, 1:1000). Nuclei were stained blue with DAPI; GFPT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meidum. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q06210
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB2800
Calculated MW Predicted, 79 kDa; observed, 75 kDa
Gene Name GFPT1
Aliases GFPT1; Glutamine--Fructose-6-Phosphate Transaminase 1; GFAT1; GFAT; GFPT; GFA; Glutamine--Fructose-6-Phosphate Aminotransferase [Isomerizing] 1; Glutamine:Fructose-6-Phosphate Amidotransferase 1; D-Fructose-6-Phosphate Amidotransferase 1; Hexosephosphate Aminotransferase 1; EC 2.6.1.16; GFAT 1; Glucosamine--Fructose-6-Phosphate Aminotransferase [Isomerizing] 1; Glutamine-Fructose-6-Phosphate Transaminase 1; CMSTA1; GFAT1m; GFPT1L; CMS12; MSLG
Immunogen A synthesized peptide derived from human GFPT1
Additional Information
Gene ID 2673
Other Names Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1, 2.6.1.16, D-fructose-6-phosphate amidotransferase 1, Glutamine:fructose-6-phosphate amidotransferase 1, GFAT 1, GFAT1, Hexosephosphate aminotransferase 1, GFPT1, GFAT, GFPT
Protein Information
Name GFPT1
Synonyms GFAT, GFPT
Function Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes BMAL1 and CRY1 (By similarity). Has a role in fine tuning the metabolic fluctuations of cytosolic UDP-GlcNAc and its effects on hyaluronan synthesis that occur during tissue remodeling (PubMed:26887390).
Tissue Location Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1298
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