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KD-Validated Anti-Cyclin B2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Cyclin B2 Rabbit Monoclonal Antibody AGI1321
    Western blotting analysis using anti-cyclin B2 antibody (Cat#61583). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin B2 antibody (Cat#61583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-Cyclin B2 Rabbit Monoclonal Antibody AGI1321
    Western blotting analysis using anti-cyclin B2 antibody (Cat#61583). Cyclin B2 expression in wild-type (WT) and cyclin B2 (CCNB2) knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin B2 antibody (Cat#61583, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • ICC - KD-Validated Anti-Cyclin B2 Rabbit Monoclonal Antibody AGI1321
    Immunocytochemical staining of HepG2 cells with anti-Cyclin B2 antibody (Cat#61583, 1:1,000). Nuclei were stained blue with DAPI; Cyclin B2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession O95067
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB2920
Calculated MW Predicted, 45 kDa; observed, 45 kDa
Gene Name CCNB2
Aliases CCNB2; Cyclin B2; HsT17299; G2/Mitotic-Specific Cyclin-B2
Immunogen A synthesized peptide derived from human Cyclin B2
Additional Information
Gene ID 9133
Other Names G2/mitotic-specific cyclin-B2, CCNB2
Protein Information
Name CCNB2
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1321
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