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KD-Validated Anti-DEK Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-DEK Rabbit Monoclonal Antibody AGI1338
    Western blotting analysis using anti-DEK antibody (Cat#AGI1338). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DEK antibody (Cat#AGI1338, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-DEK Rabbit Monoclonal Antibody AGI1338
    Western blotting analysis using anti-DEK antibody (Cat#AGI1338). DEK expression in wild type (WT) and DEK shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DEK antibody (Cat#AGI1338, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-DEK Rabbit Monoclonal Antibody AGI1338
    Flow cytometric analysis of DEK expression in HeLa cells using DEK antibody (Cat#AGI1338, 1:2,000). Green, isotype control; red, DEK.
    detail
  • ICC - KD-Validated Anti-DEK Rabbit Monoclonal Antibody AGI1338
    Immunocytochemical staining of Hela cells with DEK antibody (Cat#AGI1338, 1:1,000). Nuclei were stained blue with DAPI; DEK was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P35659
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3570
Calculated MW Predicted, 43 kDa , observed, 43 kDa
Gene Name DEK
Aliases DEK; DEK Proto-Oncogene; D6S231E; DEK Oncogene (DNA Binding); Protein DEK; DEK Oncogene
Immunogen A synthesized peptide derived from human DEK
Additional Information
Gene ID 7913
Other Names Protein DEK, DEK
Protein Information
Name DEK
Function Involved in chromatin organization.
Cellular Location Nucleus. Note=Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei
Tissue Location Ubiquitous. Expressed at relatively high levels.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1338
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