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KD-Validated Anti-LIM Homeobox 3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-LIM Homeobox 3 Rabbit Monoclonal Antibody AGI1355
    Western blotting analysis using anti-LIM homeobox 3 antibody (Cat#AGI1355). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LIM homeobox 3 antibody (Cat#AGI1355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-LIM Homeobox 3 Rabbit Monoclonal Antibody AGI1355
    Western blotting analysis using anti-LIM homeobox 3 antibody (Cat#AGI1355). LIM homeobox 3 expression in wild-type (WT) and LIM homeobox 3 (LHX3) knockdown (KD) HSHC cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LIM homeobox 3 antibody (Cat#AGI1355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-LIM Homeobox 3 Rabbit Monoclonal Antibody AGI1355
    Flow cytometric analysis of LIM homeobox 3 expression in C2C12 cells using anti-LIM homeobox 3 antibody (Cat#AGI1355, 1:2,000). Green, isotype control; red, LIM homeobox 3.
    detail
  • ICC - KD-Validated Anti-LIM Homeobox 3 Rabbit Monoclonal Antibody AGI1355
    Immunocytochemical staining of C2C12 cells with anti-LIM homeobox 3 antibody (Cat#AGI1355, 1:1,000). Nuclei were stained blue with DAPI; LIM homeobox 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9UBR4
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3075
Calculated MW Predicted, 43 kDa; observed, 43 kDa
Gene Name LHX3
Aliases LHX3; LIM Homeobox 3; LIM/Homeobox Protein Lhx3; LIM Homeobox Protein 3; LIM/Homeodomain Protein LHX3; M2-LHX3; CPHD3; LIM3
Immunogen A synthesized peptide derived from human LHX3
Additional Information
Gene ID 8022
Other Names LIM/homeobox protein Lhx3, LIM homeobox protein 3, LHX3
Protein Information
Name LHX3
Function Transcription factor. Recognizes and binds to the consensus sequence motif 5'-AATTAATTA-3' in the regulatory elements of target genes, such as glycoprotein hormones alpha chain CGA and visual system homeobox CHX10, positively modulating transcription; transcription can be co-activated by LDB2. Synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (By similarity). Required for the establishment of the specialized cells of the pituitary gland and the nervous system (PubMed:21149718). Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1 (By similarity).
Cellular Location Nucleus.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1355
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