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KD-Validated Anti-SNRPA1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-SNRPA1 Rabbit Monoclonal Antibody AGI1372
    Western blotting analysis using anti-SNRPA1 antibody (Cat#AGI1372). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SNRPA1 antibody (Cat#AGI1372, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-SNRPA1 Rabbit Monoclonal Antibody AGI1372
    Western blotting analysis using anti-SNRPA1 antibody (Cat#AGI1372). SNRPA1 expression in wild-type (WT) and SNRPA1 knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SNRPA1 antibody (Cat#AGI1372, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-SNRPA1 Rabbit Monoclonal Antibody AGI1372
    Flow cytometric analysis of SNRPA1 expression in HepG2 cells using anti-SNRPA1 antibody (Cat#AGI1372, 1:2,000). Green, isotype control; red, SNRPA1.
    detail
  • ICC - KD-Validated Anti-SNRPA1 Rabbit Monoclonal Antibody AGI1372
    Immunocytochemical staining of HepG2 cells with anti-SNRPA1 antibody (Cat#AGI1372, 1:1,000). Nuclei were stained blue with DAPI; SNRPA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P09661
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3175
Calculated MW Predicted, 28 kDa; observed, 28 kDa
Gene Name SNRPA1
Aliases SNRPA1; Small Nuclear Ribonucleoprotein Polypeptide A'; Lea1; U2A'; U2 Small Nuclear Ribonucleoprotein A'; U2 SnRNP A'; U2 Small Nuclear Ribonucleoprotein Polypeptide A'
Immunogen A synthesized peptide derived from human SNRPA1
Additional Information
Gene ID 6627
Other Names U2 small nuclear ribonucleoprotein A', U2 snRNP A', SNRPA1
Protein Information
Name SNRPA1
Function Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:27035939, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:32494006). Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA (PubMed:27035939, PubMed:32494006, PubMed:9716128).
Cellular Location Nucleus
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1372
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