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KD-Validated Anti-Thymidylate Synthase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Thymidylate Synthase Rabbit Monoclonal Antibody AGI1380
    Western blotting analysis using anti-thymidylate synthetase antibody (Cat#61673). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-thymidylate synthetase antibody (Cat#61673, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-Thymidylate Synthase Rabbit Monoclonal Antibody AGI1380
    Western blotting analysis using anti-thymidylate synthetase antibody (Cat#61673). Thymidylate synthetase expression in wild type (WT) and thymidylate synthetase (TYMS) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-thymidylate synthetase antibody (Cat#61673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-Thymidylate Synthase Rabbit Monoclonal Antibody AGI1380
    Flow cytometric analysis of Thymidylate synthetase expression in HepG2 cells using anti-Thymidylate synthetase antibody (Cat#61673, 1:2,000). Green, isotype control; red, Thymidylate synthetase.
    detail
  • ICC - KD-Validated Anti-Thymidylate Synthase Rabbit Monoclonal Antibody AGI1380
    Immunocytochemical staining of HepG2 cells with anti-Thymidylate synthetase antibody (Cat#61673, 1:1,000). Nuclei were stained blue with DAPI; Thymidylate synthetase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P04818
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3300
Calculated MW Predicted, 36 kDa; observed, 36 kDa
Gene Name TYMS
Aliases TYMS; Thymidylate Synthetase; HsT422; Tsase; TMS; TS; Thymidylate Synthase; EC 2.1.1.45; TSase; DKCD
Immunogen A synthesized peptide derived from human Thymidylate Synthase
Additional Information
Gene ID 7298
Other Names Thymidylate synthase, TS, TSase, 2.1.1.45, TYMS (HGNC:12441), TS
Protein Information
Name TYMS (HGNC:12441)
Synonyms TS
Function Catalyzes the reductive methylation of 2'-deoxyuridine 5'- monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1- carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Cellular Location Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1380
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