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KD-Validated Anti-IQGAP2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-IQGAP2 Rabbit Monoclonal Antibody AGI1382
    Western blotting analysis using anti-IQGAP2 antibody (Cat#61675). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IQGAP2 antibody (Cat#61675, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-IQGAP2 Rabbit Monoclonal Antibody AGI1382
    Western blotting analysis using anti-IQGAP2 antibody (Cat#61675). IQGAP2 expression in wild type (WT) and IQGAP2 shRNA knockdown (KD) 293T cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IQGAP2 antibody (Cat#61675, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • ICC - KD-Validated Anti-IQGAP2 Rabbit Monoclonal Antibody AGI1382
    Immunocytochemical staining of HeLa cells with IQGAP2 antibody (Cat#61675, 1:1,000). Nuclei were stained blue with DAPI; IQGAP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q13576
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB4020
Calculated MW Predicted, 181 kDa , observed, 181 kDa
Gene Name IQGAP2
Aliases IQGAP2; IQ Motif Containing GTPase Activating Protein 2; Ras GTPase-Activating-Like Protein IQGAP2
Immunogen A synthesized peptide derived from human IQGAP2
Additional Information
Gene ID 10788
Other Names Ras GTPase-activating-like protein IQGAP2, IQGAP2
Protein Information
Name IQGAP2
Function Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
Tissue Location Isoform 2 expression is enhanced in testis.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1382
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