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KD-Validated Anti-Kinesin family member 23 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Kinesin family member 23 Rabbit Monoclonal Antibody AGI1384
    Western blotting analysis using anti-Kinesin family member 23 antibody (Cat#AGI1384). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Kinesin family member 23 antibody (Cat#AGI1384, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Kinesin family member 23 Rabbit Monoclonal Antibody AGI1384
    Western blotting analysis using anti-Kinesin family member 23 antibody (Cat#AGI1384). Kinesin family member 23 expression in wild type (WT) and Kinesin family member 23 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Kinesin family member 23 antibody (Cat#AGI1384, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Kinesin family member 23 Rabbit Monoclonal Antibody AGI1384
    Flow cytometric analysis of Kinesin family member 23 expression in HepG2 cells using Kinesin family member 23 antibody (Cat#AGI1384, 1:2,000). Green, isotype control; red, Kinesin family member 23.
    detail
  • ICC - KD-Validated Anti-Kinesin family member 23 Rabbit Monoclonal Antibody AGI1384
    Immunocytochemical staining of HepG2 cells with Kinesin family member 23 antibody (Cat#AGI1384, 1:1,000). Nuclei were stained blue with DAPI; Kinesin family member 23 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q02241
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB4035
Calculated MW Predicted, 110 kDa , observed, 98 kDa
Gene Name KIF23
Aliases KIF23; Kinesin Family Member 23; MKLP1; MKLP-1; KNSL5; Kinesin-Like 5 (Mitotic Kinesin-Like Protein 1); Kinesin-Like Protein KIF23; Congenital Dyserythropoietic Anemia, Type III; Mitotic Kinesin-Like Protein 1; Kinesin-Like Protein 5; CDAIII; CDAN3A; CDAN3; CDA3; CHO1
Immunogen A synthesized peptide derived from human MKLP1
Additional Information
Gene ID 9493
Other Names Kinesin-like protein KIF23, Kinesin-like protein 5, Mitotic kinesin-like protein 1, KIF23, KNSL5, MKLP1
Protein Information
Name KIF23
Synonyms KNSL5, MKLP1
Function Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
Cellular Location Nucleus. Cytoplasm, cytoskeleton, spindle. Midbody, Midbody ring. Note=Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis
Tissue Location Widely expressed..
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1384
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