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KD-Validated Anti-UFC1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-UFC1 Rabbit Monoclonal Antibody AGI1416
    Western blotting analysis using anti-UFC1 antibody (Cat#61724). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UFC1 antibody (Cat#61724, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-UFC1 Rabbit Monoclonal Antibody AGI1416
    Western blotting analysis using anti-UFC1 antibody (Cat#61724). UFC1 expression in wild type (WT) and UFC1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UFC1 antibody (Cat#61724, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-UFC1 Rabbit Monoclonal Antibody AGI1416
    Flow cytometric analysis of UFC1 expression in HeLa cells using UFC1 antibody (Cat#61724, 1:2,000). Green, isotype control; red, UFC1.
    detail
  • ICC - KD-Validated Anti-UFC1 Rabbit Monoclonal Antibody AGI1416
    Immunocytochemical staining of HeLa cells with UFC1 antibody (Cat#61724, 1:1,000). Nuclei were stained blue with DAPI; UFC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9Y3C8
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB4245
Calculated MW Predicted, 19 kDa , observed, 19 kDa
Gene Name UFC1
Aliases UFC1; Ubiquitin-Fold Modifier Conjugating Enzyme 1; HSPC155; Ubiquitin-Fold Modifier-Conjugating Enzyme 1; Ufm1-Conjugating Enzyme 1; NEDSG
Immunogen A synthesized peptide derived from human UFC1
Additional Information
Gene ID 51506
Other Names Ubiquitin-fold modifier-conjugating enzyme 1, Ufm1-conjugating enzyme 1, UFC1 {ECO:0000303|PubMed:15071506, ECO:0000312|HGNC:HGNC:26941}
Protein Information
Name UFC1 {ECO:0000303|PubMed:15071506, ECO:0000312|HGNC:HGNC:26941}
Function E2-like enzyme which specifically catalyzes the second step in ufmylation (PubMed:15071506, PubMed:29868776, PubMed:30626644, PubMed:34588452, PubMed:35394863, PubMed:36121123, PubMed:38383789). Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage (PubMed:15071506, PubMed:29868776, PubMed:34588452, PubMed:38383789). Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed:27351204, PubMed:32160526, PubMed:35394863, PubMed:37036982, PubMed:38383789).
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$ 149.00
$ 499.00
Cat# AGI1416
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