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KD-Validated Anti-EWS RNA Binding Protein 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-EWS RNA Binding Protein 1 Rabbit Monoclonal Antibody AGI1426
    Western blotting analysis using anti-EWS RNA binding protein 1 antibody (Cat#61739). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EWS RNA binding protein 1 antibody (Cat#61739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-EWS RNA Binding Protein 1 Rabbit Monoclonal Antibody AGI1426
    Western blotting analysis using anti-EWS RNA binding protein 1 antibody (Cat#61739). EWS RNA binding protein 1 expression in wild-type (WT) and EWS RNA binding protein 1 (EWSR1) knockdown (KD) 293T cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-EWS RNA binding protein 1 antibody (Cat#61739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-EWS RNA Binding Protein 1 Rabbit Monoclonal Antibody AGI1426
    Flow cytometric analysis of EWS RNA binding protein 1 expression in HepG2 cells using anti-EWS RNA binding protein 1 antibody (Cat#61739, 1:2,000). Green, isotype control; red, EWS RNA binding protein 1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q01844
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3500
Calculated MW Predicted, 68 kDa; observed, 85 kDa
Gene Name EWSR1
Aliases EWSR1; EWS RNA Binding Protein 1; EWS; Ewing Sarcoma Breakpoint Region 1; RNA-Binding Protein EWS; Ewings Sarcoma EWS-Fli1 (Type 1) Oncogene; Ewing Sarcoma Breakpoint Region 1 Protein; EWS Oncogene; BK984G1.4; EWS-FLI1
Immunogen A synthesized peptide derived from human EWSR1
Additional Information
Gene ID 2130
Other Names RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein, EWSR1, EWS
Protein Information
Name EWSR1
Synonyms EWS
Function Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed:21256132). Might normally function as a transcriptional repressor (PubMed:10767297). EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation
Tissue Location Ubiquitous.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1426
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