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KD-Validated Anti-CREB1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CREB1 Rabbit Monoclonal Antibody AGI1433
    Western blotting analysis using anti-CREB1 antibody (Cat#61764). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#61764, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-CREB1 Rabbit Monoclonal Antibody AGI1433
    Western blotting analysis using anti-CREB1 antibody (Cat#61764). CREB1 expression in wild type (WT) and CREB1 knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CREB1 antibody (Cat#61764, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-CREB1 Rabbit Monoclonal Antibody AGI1433
    Flow cytometric analysis of CREB1 expression in HepG2 cells using anti-CREB1 antibody (Cat#61764, 1:2,000). Green, isotype control; red, CREB1.
    detail
  • ICC - KD-Validated Anti-CREB1 Rabbit Monoclonal Antibody AGI1433
    Immunocytochemical staining of HepG2 cells with anti-CREB1 antibody (Cat#61764, 1:1,000). Nuclei were stained blue with DAPI; CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P16220
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3650
Calculated MW Predicted, 35 kDa; observed, 43-46 kDa
Gene Name CREB1
Aliases CAMP Responsive Element Binding Protein 1; Cyclic AMP-Responsive Element-Binding Protein 1; CREB-1; Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein CREB; Cyclic Adenosine 3',5'-Monophosphate Response Element Binding Protein; CAMP-Responsive Element-Binding Protein 1; CAMP-Response Element-Binding Protein-1; Active Transcription Factor CREB; Transactivator Protein; CREB
Immunogen A synthesized peptide derived from human CREB
Additional Information
Gene ID 1385
Other Names Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Protein Information
Name CREB1
Function Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed:14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00312, ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12552083}
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1433
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