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KD-Validated Anti-Cystatin C Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Cystatin C Rabbit Monoclonal Antibody AGI1440
    Western blotting analysis using anti-Cystatin C antibody (Cat#61773). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cystatin C antibody (Cat#61773, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Cystatin C Rabbit Monoclonal Antibody AGI1440
    Western blotting analysis using anti-Cystatin C antibody (Cat#61773).Cystatin C expression in wild type (WT) and cystatin C shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Cystatin C antibody (Cat#61773, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • ICC - KD-Validated Anti-Cystatin C Rabbit Monoclonal Antibody AGI1440
    Immunocytochemical staining of HT-1080 cells with Cystatin C antibody (Cat#61773, 1:1,000). Nuclei were stained blue with DAPI; Cystatin C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P01034
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 1845
Calculated MW Predicted, 16 kDa , observed, 14 kDa
Gene Name CST3
Aliases CST3; Cystatin C; Neuroendocrine Basic Polypeptide; Post-Gamma-Globulin; Gamma-Trace; Cystatin-C; Cystatin C (Amyloid Angiopathy And Cerebral Hemorrhage); Epididymis Secretory Protein Li; BA218C14.4 (Cystatin C); Cystatin 3; Cystatin-3; HEL-S-2 3; ARMD11
Immunogen A synthesized peptide derived from human Cystatin C
Additional Information
Gene ID 1471
Other Names Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3
Protein Information
Name CST3
Function As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Cellular Location Secreted.
Tissue Location Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1440
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