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KD-Validated Anti-HAUS7 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-HAUS7 Rabbit Monoclonal Antibody AGI1446
    Western blotting analysis using anti-HAUS7 antibody (Cat#AGI1446). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HAUS7 antibody (Cat#AGI1446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-HAUS7 Rabbit Monoclonal Antibody AGI1446
    Western blotting analysis using anti-HAUS7 antibody (Cat#AGI1446). HAUS7 expression in wild type (WT) and HAUS7 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HAUS7 antibody (Cat#AGI1446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-HAUS7 Rabbit Monoclonal Antibody AGI1446
    Flow cytometric analysis of HAUS7 expression in HepG2 cells using HAUS7 antibody (Cat#AGI1446, 1:2,000). Green, isotype control; red, HAUS7.
    detail
  • ICC - KD-Validated Anti-HAUS7 Rabbit Monoclonal Antibody AGI1446
    Immunocytochemical staining of HepG2 cells with HAUS7 antibody (Cat#AGI1446, 1:1,000). Nuclei were stained blue with DAPI; HAUS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q99871
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB4485
Calculated MW Predicted, 40 kDa , observed, 37 kDa
Gene Name HAUS7
Aliases HAUS7; HAUS Augmin Like Complex Subunit 7; UIP1; UCHL5IP; 26S Proteasome-Associated UCH37-Interacting Protein 1; HAUS Augmin-Like Complex Subunit 7; X-Linked Protein STS1769; 26S Proteasome-Associated UCH Interacting Protein 1; UCH37 Interacting Protein 1; UCHL5 Interacting Protein; UCHL5-Interacting Protein
Immunogen A synthesized peptide derived from human UCHL5IP
Additional Information
Gene ID 55559
Other Names HAUS augmin-like complex subunit 7, 26S proteasome-associated UCH37-interacting protein 1, UCHL5-interacting protein, X-linked protein STS1769, HAUS7, UCHL5IP, UIP1
Protein Information
Name HAUS7
Synonyms UCHL5IP, UIP1
Function Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Localizes to interphase centrosomes and to mitotic spindle microtubules.
Tissue Location Detected in spleen, thymus, testis, ovary, small intestine and colon, with highest levels of expression in testis and ovary.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1446
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