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KD-Validated Anti-AGPS Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-AGPS Rabbit Monoclonal Antibody AGI1458
    Western blotting analysis using anti-alkylglycerone phosphate synthase antibody (Cat#AGI1458). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alkylglycerone phosphate synthase antibody (Cat#AGI1458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-AGPS Rabbit Monoclonal Antibody AGI1458
    Western blotting analysis using anti-alkylglycerone phosphate synthase antibody (Cat#AGI1458). Alkylglycerone phosphate synthase expression in wild-type (WT) and alkylglycerone phosphate synthase (AGPS) shRNA knockdown (KD) HepG2 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-alkylglycerone phosphate synthase antibody (Cat#AGI1458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-AGPS Rabbit Monoclonal Antibody AGI1458
    Flow cytometric analysis of Alkylglycerone phosphate synthase expression in HeLa cells using anti-Alkylglycerone phosphate synthase antibody (Cat#AGI1458, 1:2,000). Green, isotype control; red, Alkylglycerone phosphate synthase.
    detail
  • ICC - KD-Validated Anti-AGPS Rabbit Monoclonal Antibody AGI1458
    Immunocytochemical staining of Hela cells with anti-Alkylglycerone phosphate synthase antibody (Cat#AGI1458, 1:1,000). Nuclei were stained blue with DAPI; Alkylglycerone phosphate synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O00116
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3850
Calculated MW Predicted, 73 kDa; observed, 73 kDa
Gene Name AGPS
Aliases AGPS; Alkylglycerone Phosphate Synthase; ALDHPSY; ADHAPS; ADAP-S; ADAS; ADPS; Alkyldihydroxyacetonephosphate Synthase, Peroxisomal; Aging-Associated Gene 5 Protein; Alkyl-DHAP Synthase; EC 2.5.1.26; Alkylglycerone-Phosphate Synthase; Aging-Associated Protein 5; RCDP3
Immunogen Recombinant protein of human Alkyl-DHAP synthase
Additional Information
Gene ID 8540
Other Names Alkyldihydroxyacetonephosphate synthase, peroxisomal, Alkyl-DHAP synthase, 2.5.1.26, Aging-associated gene 5 protein, Alkylglycerone-phosphate synthase, AGPS
Protein Information
Name AGPS
Function Catalyzes the exchange of the acyl chain in acyl- dihydroxyacetonephosphate (acyl-DHAP) for a long chain fatty alcohol, yielding the first ether linked intermediate, i.e. alkyl- dihydroxyacetonephosphate (alkyl-DHAP), in the pathway of ether lipid biosynthesis.
Cellular Location Peroxisome membrane {ECO:0000250|UniProtKB:P97275}. Peroxisome {ECO:0000250|UniProtKB:P97275}
Research Areas
Citations (0)
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$ 399.20
$ 149.00
Cat# AGI1458
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