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KD-Validated Anti-AVEN Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-AVEN Rabbit Monoclonal Antibody AGI1461
    Western blotting analysis using anti-apoptosis and caspase activation inhibitor antibody (Cat#AGI1461). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-apoptosis and caspase activation inhibitor antibody (Cat#AGI1461, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-AVEN Rabbit Monoclonal Antibody AGI1461
    Western blotting analysis using anti-apoptosis and caspase activation inhibitor antibody (Cat#AGI1461). Apoptosis and caspase activation inhibitor expression in wild-type (WT) and apoptosis and caspase activation inhibitor (AVEN) shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-apoptosis and caspase activation inhibitor antibody (Cat#AGI1461, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-AVEN Rabbit Monoclonal Antibody AGI1461
    Flow cytometric analysis of Apoptosis and caspase activation inhibitor expression in HepG2 cells using anti-Apoptosis and caspase activation inhibitor antibody (Cat#AGI1461, 1:2,000). Green, isotype control; red, Apoptosis and caspase activation inhibitor.
    detail
  • ICC - KD-Validated Anti-AVEN Rabbit Monoclonal Antibody AGI1461
    Immunocytochemical staining of HepG2 cells with anti-Apoptosis and caspase activation inhibitor antibody (Cat#AGI1461, 1:1,000). Nuclei were stained blue with DAPI; Apoptosis and caspase activation inhibitor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9NQS1
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3870
Calculated MW Predicted, 39 kDa; observed, 50 kDa
Gene Name AVEN
Aliases AVEN; Apoptosis And Caspase Activation Inhibitor; Cell Death Regulator Aven; PDCD12; Programmed Cell Death 12; Apoptosis, Caspase Activation Inhibitor
Immunogen A synthesized peptide derived from human AVEN
Additional Information
Gene ID 57099
Other Names Cell death regulator Aven, AVEN
Protein Information
Name AVEN
Function Protects against apoptosis mediated by Apaf-1.
Cellular Location Endomembrane system; Peripheral membrane protein. Note=Associated with intracellular membranes
Tissue Location Highly expressed in testis, ovary, thymus, prostate, spleen, small intestine, colon, heart, skeletal muscle, liver, kidney and pancreas
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1461
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