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KD-Validated Anti-ATP1B3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-ATP1B3 Rabbit Monoclonal Antibody AGI1474
    Western blotting analysis using anti-ATP1B3 antibody (Cat#61848). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP1B3 antibody (Cat#61848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-ATP1B3 Rabbit Monoclonal Antibody AGI1474
    Western blotting analysis using anti-ATP1B3 antibody (Cat#61848). ATP1B3 expression in wild type (WT) and ATP1B3 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP1B3 antibody (Cat#61848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P54709
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB4740
Calculated MW Predicted, 32 kDa , observed, 37 kDa
Gene Name ATP1B3
Aliases ATP1B3; ATPase Na+/K+ Transporting Subunit Beta 3; Sodium/Potassium-Transporting ATPase Subunit Beta-3; CD298; Sodium-Potassium ATPase Subunit Beta 3 (Non-Catalytic); Sodium/Potassium-Dependent ATPase Subunit Beta-3; ATPase, Na+/K+ Transporting, Beta 3 Polypeptide; Sodium Pump Subunit Beta-3; FLJ29027; ATPB-3; Sodium/Potassium-Transporting ATPase Beta-3 Chain; Na, K-ATPase Beta-3 Polypeptide; CD298 Antigen
Immunogen A synthesized peptide derived from human ATP1B3
Additional Information
Gene ID 483
Other Names Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, ATPB-3, CD298, ATP1B3
Protein Information
Name ATP1B3
Function This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Cellular Location Apical cell membrane {ECO:0000250|UniProtKB:Q63377}; Single-pass type II membrane protein. Basolateral cell membrane {ECO:0000250|UniProtKB:Q63377}; Single-pass type II membrane protein. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1474
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