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KD-Validated Anti-Early endosome antigen 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Early endosome antigen 1 Rabbit Monoclonal Antibody AGI1513
    Western blotting analysis using anti-Early endosome antigen 1 antibody (Cat#AGI1513). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Early endosome antigen 1 antibody (Cat#AGI1513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Early endosome antigen 1 Rabbit Monoclonal Antibody AGI1513
    Western blotting analysis using anti-Early endosome antigen 1 antibody (Cat#AGI1513). Early endosome antigen 1 expression in wild type (WT) and Early endosome antigen 1 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Early endosome antigen 1 antibody (Cat#AGI1513, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Early endosome antigen 1 Rabbit Monoclonal Antibody AGI1513
    Flow cytometric analysis of Early endosome antigen 1 expression in HT-1080 cells using Early endosome antigen 1 antibody (Cat#AGI1513, 1:2,000). Green, isotype control; red, Early endosome antigen 1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q15075
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB5260
Calculated MW Predicted, 162 kDa, observed, 148 kDa
Gene Name EEA1
Aliases EEA1; Early Endosome Antigen 1; ZFYVE2; Zinc Finger FYVE Domain-Containing Protein; Endosome-Associated Protein P162; Early Endosome Antigen 1, 162kD; Early Endosome-Associated Protein; MSTP105; MST105
Immunogen A synthesized peptide derived from human EEA1
Additional Information
Gene ID 8411
Other Names Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2, EEA1, ZFYVE2
Protein Information
Name EEA1
Synonyms ZFYVE2
Function Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Cellular Location Cytoplasm. Early endosome membrane; Peripheral membrane protein
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1513
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