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KD-Validated Anti-ELP4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-ELP4 Rabbit Monoclonal Antibody AGI1516
    Western blotting analysis using anti-ELP4 antibody (Cat#AGI1516). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ELP4 antibody (Cat#AGI1516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-ELP4 Rabbit Monoclonal Antibody AGI1516
    Western blotting analysis using anti-ELP4 antibody (Cat#AGI1516). ELP4 expression in wild type (WT) and ELP4 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ELP4 antibody (Cat#AGI1516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-ELP4 Rabbit Monoclonal Antibody AGI1516
    Flow cytometric analysis of ELP4 expression in C2C12 cells using ELP4 antibody (Cat#AGI1516, 1:2,000). Green, isotype control; red, ELP4.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q96EB1
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB5310
Calculated MW Predicted, 47 kDa , observed, 46 kDa
Gene Name ELP4
Aliases ELP4; Elongator Acetyltransferase Complex Subunit 4; PAXNEB; Elongator Complex Protein 4; PAX6 Neighbor Gene Protein; C11orf19; HELP4; Elongation Protein 4 Homolog (S. Cerevisiae); Chromosome 11 Open Reading Frame 19; Elongation Protein 4 Homolog; DJ68P15A.1; C11ORF19; PAX6NEB; AN2; AN
Immunogen A synthesized peptide derived from human ELP4
Additional Information
Gene ID 26610
Other Names Elongator complex protein 4, hELP4, PAX6 neighbor gene protein, ELP4, C11orf19, PAXNEB
Protein Information
Name ELP4
Synonyms C11orf19, PAXNEB
Function Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed:29332244). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (PubMed:29332244).
Cellular Location Cytoplasm. Nucleus
Tissue Location Widely expressed..
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1516
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