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KD-Validated Anti-Galactosidase alpha Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Galactosidase alpha Rabbit Monoclonal Antibody AGI1523
    Western blotting analysis using anti-Galactosidase alpha antibody (Cat#62009). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Galactosidase alpha antibody (Cat#62009, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Galactosidase alpha Rabbit Monoclonal Antibody AGI1523
    Western blotting analysis using anti-Galactosidase alpha antibody (Cat#62009). Galactosidase alpha expression in wild type (WT) and Galactosidase alpha shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Galactosidase alpha antibody (Cat#62009, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Galactosidase alpha Rabbit Monoclonal Antibody AGI1523
    Flow cytometric analysis of Galactosidase alpha expression in HeLa cells using Galactosidase alpha antibody (Cat#62009, 1:2,000). Green, isotype control; red, Galactosidase alpha.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P06280
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB5545
Calculated MW Predicted, 49 kDa , observed, 46 kDa
Gene Name GLA
Aliases GLA; Galactosidase Alpha; GALA; Galactosylgalactosylglucosylceramidase GLA; Alpha-D-Galactosidase A; Alpha-Galactosidase A; EC 3.2.1.22; Melibiase; Alpha-D-Galactoside Galactohydrolase 1; Alpha-D-Galactoside Galactohydrolase; Agalsidase Alfa; Alpha-Gal A; Agalsidase; EC 3.2.1
Immunogen A synthesized peptide derived from human Galactosidase alpha
Additional Information
Gene ID 2717
Other Names Alpha-galactosidase A, 3.2.1.22, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Galactosylgalactosylglucosylceramidase GLA, Melibiase, Agalsidase, GLA (HGNC:4296)
Protein Information
Name GLA (HGNC:4296)
Function Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome.
Cellular Location Lysosome.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1523
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