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KD-Validated Anti-Glutamic-oxaloacetic transaminase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Glutamic-oxaloacetic transaminase 1 Rabbit Monoclonal Antibody AGI1528
    Western blotting analysis using anti-Glutamic-oxaloacetic transaminase 1 antibody (Cat#62017). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glutamic-oxaloacetic transaminase 1 antibody (Cat#62017, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Glutamic-oxaloacetic transaminase 1 Rabbit Monoclonal Antibody AGI1528
    Western blotting analysis using anti-Glutamic-oxaloacetic transaminase 1 antibody (Cat#62017). Glutamic-oxaloacetic transaminase 1 expression in wild type (WT) and Glutamic-oxaloacetic transaminase 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Glutamic-oxaloacetic transaminase 1 antibody (Cat#62017, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Glutamic-oxaloacetic transaminase 1 Rabbit Monoclonal Antibody AGI1528
    Flow cytometric analysis of Glutamic-oxaloacetic transaminase 1 expression in C2C12 cells using Glutamic-oxaloacetic transaminase 1 antibody (Cat#62017, 1:2,000). Green, isotype control; red, Glutamic-oxaloacetic transaminase 1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P17174
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB5585
Calculated MW Predicted, 46 kDa , observed, 47 kDa
Gene Name GOT1
Aliases GOT1; Glutamic-Oxaloacetic Transaminase 1; Glutamate Oxaloacetate Transaminase 1; AST1; SGOT; AST; Glutamic-Oxaloacetic Transaminase 1, Soluble; Aspartate Aminotransferase, Cytoplasmic; Cysteine Aminotransferase, Cytoplasmic; Cysteine Transaminase, Cytoplasmic; Aspartate Aminotransferase 1; Aspartate Transaminase 1; Transaminase A; EC 2.6.1.1; CAspAT; CCAT; Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1); Testis Secretory Sperm-Binding Protein Li 196a; Growth-Inhibiting Protein 18; EC 2.6.1.3; ASTQTL1; GIG18
Immunogen A synthesized peptide derived from human Aspartate Aminotransferase
Additional Information
Gene ID 2805
Other Names Aspartate aminotransferase, cytoplasmic, cAspAT, 2.6.1.1, 2.6.1.3, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, cCAT, Glutamate oxaloacetate transaminase 1, Transaminase A, GOT1 (HGNC:4432)
Protein Information
Name GOT1 (HGNC:4432)
Function Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed:21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2- aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed:27827456).
Cellular Location Cytoplasm.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1528
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