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KD-Validated Anti-Phospho-GATA3(S308) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Phospho-GATA3(S308) Rabbit Monoclonal Antibody AGI1537
    Western blotting analysis using anti-Phospho-GATA3(S308) antibody (Cat#62044). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-GATA3(S308) antibody (Cat#62044, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Phospho-GATA3(S308) Rabbit Monoclonal Antibody AGI1537
    Western blotting analysis using anti-Phospho-GATA3(S308) antibody (Cat#62044). Phospho-GATA3(S308) expression in wild type (WT) and Phospho-GATA3(S308) shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-GATA3(S308) antibody (Cat#62044, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Phospho-GATA3(S308) Rabbit Monoclonal Antibody AGI1537
    Flow cytometric analysis of EPH receptor B1 expression in HT-1080 cells using anti-EPH receptor B1 antibody (Cat#, 1:2,000). Green, isotype control; red, EPH receptor B1.
    detail
  • ICC - KD-Validated Anti-Phospho-GATA3(S308) Rabbit Monoclonal Antibody AGI1537
    Immunocytochemical staining of HepG2 cells with anti-phospho-GATA3 (S308) antibody (Cat#62044, 1:1,000). Nuclei were stained blue with DAPI; Phospho-GATA3 (S308) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium: Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P23771
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB2360
Calculated MW Predicted, 48 kDa , observed, 48 kDa
Gene Name GATA3
Aliases GATA3; GATA Binding Protein 3; HDR; Trans-Acting T-Cell-Specific Transcription Factor GATA-3; GATA-Binding Factor 3; GATA-Binding Protein 3; HDRS
Immunogen A synthesized peptide derived from human Phospho-GATA3(S308)
Additional Information
Gene ID 2625
Other Names Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3
Protein Information
Name GATA3
Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity). Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.
Cellular Location Nucleus.
Tissue Location T-cells and endothelial cells.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1537
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