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KD-Validated Anti-Keratin 4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Keratin 4 Rabbit Monoclonal Antibody AGI1551
    Western blotting analysis using anti-Keratin 4 antibody (Cat#AGI1551). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 4 antibody (Cat#AGI1551, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Keratin 4 Rabbit Monoclonal Antibody AGI1551
    Western blotting analysis using anti-Keratin 4 antibody (Cat#AGI1551). Keratin 4 expression in wild type (WT) and Keratin 4 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Keratin 4 antibody (Cat#AGI1551, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Keratin 4 Rabbit Monoclonal Antibody AGI1551
    Flow cytometric analysis of Keratin 4 expression in H9c2 cells using Keratin 4 antibody (Cat#AGI1551, 1:2,000). Green, isotype control; red, Keratin 4.
    detail
  • ICC - KD-Validated Anti-Keratin 4 Rabbit Monoclonal Antibody AGI1551
    Immunocytochemical staining of H9C2 cells with anti-Keratin 4 antibody (Cat#AGI1551, 1:1,000). Nuclei were stained blue with DAPI; Keratin 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P19013
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB6150
Calculated MW Predicted, 56 kDa , observed , 57 kDa
Gene Name KRT4
Aliases KRT4; Keratin 4; CYK4; K4; Keratin, Type II Cytoskeletal 4; CK4; Type-II Keratin Kb4; Cytokeratin 4; CK-4; Keratin 4, Type II; Cytokeratin-4; Keratin-4; WSN1
Immunogen A synthesized peptide derived from human Cytokeratin 4
Additional Information
Gene ID 3851
Other Names Keratin, type II cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, Type-II keratin Kb4, KRT4, CYK4
Protein Information
Name KRT4
Synonyms CYK4
Tissue Location Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1551
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