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KD-Validated Anti-JUNB Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-JUNB Rabbit Monoclonal Antibody AGI1553
    Western blotting analysis using anti-JUNB antibody (Cat#AGI1553). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-JUNB antibody (Cat#AGI1553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-JUNB Rabbit Monoclonal Antibody AGI1553
    Western blotting analysis using anti-JunB antibody (Cat#AGI1553). JunB expression in wild type (WT) and JunB shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-JunB antibody (Cat#AGI1553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-JUNB Rabbit Monoclonal Antibody AGI1553
    Flow cytometric analysis of JUNB expression in HepG2 cells using JUNB antibody (Cat#AGI1553, 1:2,000). Green, isotype control; red, JUNB.
    detail
  • ICC - KD-Validated Anti-JUNB Rabbit Monoclonal Antibody AGI1553
    Immunocytochemical staining of HepG2 cells with anti-JUNB antibody (Cat#AGI1553, 1:1,000). Nuclei were stained blue with DAPI; JUNB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P17275
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB6175
Calculated MW Predicted, 36 kDa , observed , 43 kDa
Gene Name JUNB
Aliases JUNB; JunB Proto-Oncogene, AP-1 Transcription Factor Subunit; Transcription Factor AP-1 Subunit JunB; Transcription Factor JunB; Transcription Factor Jun-B; Jun B Proto-Oncogene; Activator Protein 1; AP-1
Immunogen A synthesized peptide derived from human JunB
Additional Information
Gene ID 3726
Other Names Transcription factor JunB, Transcription factor AP-1 subunit JunB, JUNB
Protein Information
Name JUNB
Function Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1553
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