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KD-Validated Anti-Phospho-JunD (S255) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Phospho-JunD (S255) Rabbit Monoclonal Antibody AGI1570
    Western blotting analysis using anti-Phospho-JunD (S255) antibody (Cat#62216). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-JunD (S255) antibody (Cat#62216, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Phospho-JunD (S255) Rabbit Monoclonal Antibody AGI1570
    Western blotting analysis using anti-Phospho-JunD (S255) antibody (Cat#62216). Phospho-JunD (S255) expression in wild type (WT) and Phospho-JunD (S255) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-JunD (S255) antibody (Cat#62216, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-Phospho-JunD (S255) Rabbit Monoclonal Antibody AGI1570
    Flow cytometric analysis of Phospho-JunD (S255) expression in HepG2 cells using Phospho-JunD (S255) antibody (Cat#62216, 1:2,000). Green, isotype control; red, Phospho-JunD (S255).
    detail
  • ICC - KD-Validated Anti-Phospho-JunD (S255) Rabbit Monoclonal Antibody AGI1570
    Immunocytochemical staining of HepG2 cells with anti-Phospho-JunD (S255) antibody (Cat#62216, 1:1,000). Nuclei were stained blue with DAPI; Phospho-JunD (S255) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P17535
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB6555
Calculated MW Predicted, 35 kDa , observed , 38,42 kDa
Gene Name JUND
Aliases JUND; JunD Proto-Oncogene, AP-1 Transcription Factor Subunit; AP-1; Transcription Factor AP-1 Subunit JunD; Transcription Factor Jun-D; Transcription Factor JunD; Activator Protein 1; Jun D Proto-Oncogene; JunD-FL Isoform
Immunogen A synthesized peptide derived from human JunD
Additional Information
Gene ID 3727
Other Names Transcription factor JunD, Transcription factor AP-1 subunit JunD, JUND
Protein Information
Name JUND
Function Transcription factor binding AP-1 sites (PubMed:9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed:28981703, PubMed:9989505).
Cellular Location Nucleus.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1570
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