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KD-Validated Anti-Musashi RNA Binding Protein 2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Musashi RNA Binding Protein 2 Rabbit Monoclonal Antibody AGI1590
    Western blotting analysis using anti-MSI2 antibody (Cat#AGI1590). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MSI2 antibody (Cat#AGI1590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Musashi RNA Binding Protein 2 Rabbit Monoclonal Antibody AGI1590
    Western blotting analysis using anti-MSI2 antibody (Cat#AGI1590). MSI2 expression in wild type (WT) and MSI2 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MSI2 antibody (Cat#AGI1590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Musashi RNA Binding Protein 2 Rabbit Monoclonal Antibody AGI1590
    Flow cytometric analysis of MSI2 expression in HepG2 cells using MSI2 antibody (Cat#AGI1590, 1:2,000). Green, isotype control; red, MSI2.
    detail
  • ICC - KD-Validated Anti-Musashi RNA Binding Protein 2 Rabbit Monoclonal Antibody AGI1590
    Immunocytochemical staining of HepG2 cells with anti-MSI2 antibody (Cat#AGI1590, 1:1,000). Nuclei were stained blue with DAPI; MSI2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q96DH6
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB225
Calculated MW Predicted, 35 kDa , observed , 35 kDa
Gene Name MSI2
Aliases Musashi RNA Binding Protein 2; RNA-Binding Protein Musashi Homolog 2; Musashi-2; Musashi Homolog 2 (Drosophila); Musashi Homolog 2; MSI2H
Immunogen A synthesized peptide derived from human MSI2
Additional Information
Gene ID 124540
Other Names RNA-binding protein Musashi homolog 2, Musashi-2, MSI2
Protein Information
Name MSI2
Function RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
Cellular Location Cytoplasm. Note=Associated with polysomes.
Tissue Location Ubiquitous; detected at low levels.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1590
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