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KD-Validated Anti-MAP3K2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-MAP3K2 Rabbit Monoclonal Antibody AGI1593
    Western blotting analysis using anti-MEKK2 antibody (Cat#AGI1593). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEKK2 antibody (Cat#AGI1593, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-MAP3K2 Rabbit Monoclonal Antibody AGI1593
    Western blotting analysis using anti-MEKK2 antibody (Cat#AGI1593). MEKK2 expression in wild type (WT) and MEKK2 shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MEKK2 antibody (Cat#AGI1593, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-MAP3K2 Rabbit Monoclonal Antibody AGI1593
    Flow cytometric analysis of MEKK2 expression in HepG2 cells using MEKK2 antibody (Cat#AGI1593, 1:2,000). Green, isotype control; red, MEKK2.
    detail
  • ICC - KD-Validated Anti-MAP3K2 Rabbit Monoclonal Antibody AGI1593
    Immunocytochemical staining of Hela cells with anti-MEKK2 antibody (Cat#AGI1593, 1:1,000). Nuclei were stained blue with DAPI; MEKK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9Y2U5
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB260
Calculated MW Predicted, 70 kDa , observed , 70 kDa
Gene Name MAP3K2
Aliases Mitogen-Activated Protein Kinase Kinase Kinase 2; MEKK2B; MEKK2; MAPK/ERK Kinase Kinase 2; MAP/ERK Kinase Kinase 2; MEK Kinase 2; MEKK 2; EC 2.7.11.25; MAPKKK2
Immunogen A synthesized peptide derived from human MEKK2
Additional Information
Gene ID 10746
Other Names Mitogen-activated protein kinase kinase kinase 2, 2.7.11.25, MAPK/ERK kinase kinase 2, MEK kinase 2, MEKK 2, MAP3K2, MAPKKK2, MEKK2
Protein Information
Name MAP3K2
Synonyms MAPKKK2, MEKK2
Function Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and- run dynamics.
Cellular Location Cytoplasm. Nucleus. Note=Upon EGF stimulation, translocates into the nucleus
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1593
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