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KD-Validated Anti-Pre-MRNA Processing Factor 4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Pre-MRNA Processing Factor 4 Rabbit Monoclonal Antibody AGI1639
    Western blotting analysis using anti-PRPF4 antibody (Cat#AGI1639). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PRPF4 antibody (Cat#AGI1639, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Pre-MRNA Processing Factor 4 Rabbit Monoclonal Antibody AGI1639
    Western blotting analysis using anti-PRPF4 antibody (Cat#AGI1639). PRPF4 expression in wild type (WT) and PRPF4 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PRPF4 antibody (Cat#AGI1639, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Pre-MRNA Processing Factor 4 Rabbit Monoclonal Antibody AGI1639
    Flow cytometric analysis of PRPF4 expression in HepG2 cells using anti-PRPF4 antibody (Cat#AGI1639, 1:2,000). Green, isotype control; red, PRPF4.
    detail
  • ICC - KD-Validated Anti-Pre-MRNA Processing Factor 4 Rabbit Monoclonal Antibody AGI1639
    Immunocytochemical staining of HepG2 cells with anti-PRPF4 antibody (Cat#AGI1639, 1:1,000). Nuclei were stained blue with DAPI; PRPF4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O43172
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB975
Calculated MW Predicted, 58 kDa , observed , 58 kDa
Gene Name PRPF4
Aliases Pre-MRNA Processing Factor 4; PRP4; U4/U6 Small Nuclear Ribonucleoprotein Prp4; SNRNP60; HPRP4P; Prp4p; HPRP4; PRP4/STK/WD Splicing Factor; U4/U6 SnRNP 60 KDa Protein; WD Splicing Factor Prp4; PRP4 Homolog; PRP4 Pre-MRNA Processing Factor 4 Homolog (Yeast); PRP4 Pre-MRNA Processing Factor 4 Homolog; HPrp4; RP70
Immunogen A synthesized peptide derived from human PRPF4
Additional Information
Gene ID 9128
Other Names U4/U6 small nuclear ribonucleoprotein Prp4, PRP4 homolog, hPrp4, U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4, PRPF4, PRP4
Protein Information
Name PRPF4
Synonyms PRP4
Function Plays a role in pre-mRNA splicing as component of the U4/U6- U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
Cellular Location Nucleus. Nucleus speckle
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1639
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