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KD-Validated Anti-RAD23A Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-RAD23A Rabbit Monoclonal Antibody AGI1649
    Western blotting analysis using anti-RAD23A antibody (Cat#AGI1649). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD23A antibody (Cat#AGI1649, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-RAD23A Rabbit Monoclonal Antibody AGI1649
    Western blotting analysis using anti-RAD23A antibody (Cat#AGI1649). RAD23A expression in wild type (WT) and RAD23A shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAD23A antibody (Cat#AGI1649, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-RAD23A Rabbit Monoclonal Antibody AGI1649
    Flow cytometric analysis of RAD23A expression in HepG2 cells using anti-RAD23A antibody (Cat#AGI1649, 1:2,000). Green, isotype control; red, RAD23A.
    detail
  • ICC - KD-Validated Anti-RAD23A Rabbit Monoclonal Antibody AGI1649
    Immunocytochemical staining of HepG2 cells with anti-RAD23A antibody (Cat#AGI1649, 1:1,000). Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P54725
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1080
Calculated MW Predicted, 40 kDa , observed , 55 kDa
Gene Name RAD23A
Aliases RAD23 Homolog A, Nucleotide Excision Repair Protein; HHR23A; UV Excision Repair Protein RAD23 Homolog A; RAD23, Yeast Homolog, A; MGC111083; HR23A; RAD23 (S. Cerevisiae) Homolog A; RAD23 Homolog A (S. Cerevisiae)
Immunogen A synthesized peptide derived from human RAD23A
Additional Information
Gene ID 5886
Other Names UV excision repair protein RAD23 homolog A, HR23A, hHR23A, RAD23A
Protein Information
Name RAD23A
Function Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'- linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
Cellular Location Nucleus.
Research Areas
Citations (0)
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$ 399.20
$ 149.00
Cat# AGI1649
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