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KD-Validated Anti-SAMM50 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-SAMM50 Rabbit Monoclonal Antibody AGI1654
    Western blotting analysis using anti-SAMM50 antibody (Cat#62499). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SAMM50 antibody (Cat#62499, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-SAMM50 Rabbit Monoclonal Antibody AGI1654
    Western blotting analysis using anti-SAMM50 antibody (Cat#62499). SAMM50 expression in wild type (WT) and SAMM50 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SAMM50 antibody (Cat#62499, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-SAMM50 Rabbit Monoclonal Antibody AGI1654
    Flow cytometric analysis of SAMM50 expression in HeLa cells using SAMM50 antibody (Cat#62499, 1:2,000). Green, isotype control; red, SAMM50.
    detail
  • ICC - KD-Validated Anti-SAMM50 Rabbit Monoclonal Antibody AGI1654
    Immunocytochemical staining of HeLa cells with anti-SAMM50 antibody (Cat#62499, 1:1,000). Nuclei were stained blue with DAPI; SAMM50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9Y512
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1135
Calculated MW Predicted, 52 kDa , observed , 52 kDa
Gene Name SAMM50
Aliases SAMM50 Sorting And Assembly Machinery Component; TRG-3; SAM50; YNL026W; CGI-51; OMP85; TOB55; Sorting And Assembly Machinery Component 50 Homolog; Transformation-Related Gene 3 Protein; Sorting And Assembly Machinery Component 50 Homolog (S. Cerevisiae); Sorting And Assembly Machinery 50kDa
Immunogen A synthesized peptide derived from human SAMM50
Additional Information
Gene ID 25813
Other Names Sorting and assembly machinery component 50 homolog, Transformation-related gene 3 protein, TRG-3, SAMM50, SAM50
Protein Information
Name SAMM50
Synonyms SAM50
Function Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes (PubMed:22252321, PubMed:25781180). Required for the assembly of TOMM40 into the TOM complex (PubMed:15644312).
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein. Cytoplasm {ECO:0000250|UniProtKB:Q6AXV4}. Mitochondrion
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1654
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