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KD-Validated Anti-PPP2R5E Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PPP2R5E Rabbit Monoclonal Antibody AGI1661
    Western blotting analysis using anti-PPP2R5E antibody (Cat#62517). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP2R5E antibody (Cat#62517, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
    detail
  • WB - KD-Validated Anti-PPP2R5E Rabbit Monoclonal Antibody AGI1661
    Western blotting analysis using anti-PPP2R5E antibody (Cat#62517). PPP2R5E expression in wild type (WT) and PPP2R5E shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPP2R5E antibody (Cat#62517, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-PPP2R5E Rabbit Monoclonal Antibody AGI1661
    Flow cytometric analysis of PPP2R5E expression in HepG2 cells using anti-PPP2R5E antibody (Cat#62517, 1:2,000). Green, isotype control; red, PPP2R5E.
    detail
  • ICC - KD-Validated Anti-PPP2R5E Rabbit Monoclonal Antibody AGI1661
    Immunocytochemical staining of HepG2 cells with anti-PPP2R5E antibody (Cat#62517, 1:1,000). Nuclei were stained blue with DAPI; PPP2R5E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q16537
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1215
Calculated MW Predicted, 55 kDa , observed , 50 kDa
Gene Name PPP2R5E
Aliases Protein Phosphatase 2 Regulatory Subunit B'Epsilon; B56epsilon; B56E; Serine/Threonine-Protein Phosphatase 2A 56 KDa Regulatory Subunit Epsilon Isoform; Serine/Threonine Protein Phosphatase 2A, 56 KDa Regulatory Subunit, Epsilon; Protein Phosphatase 2, Regulatory Subunit B (B56), Epsilon Isoform; Protein Phosphatase 2 Regulatory Subunit B', Epsilon; PP2A B Subunit Isoform PR61-Epsilon; PP2A B Subunit Isoform B56-Epsilon; PP2A B Subunit Isoform B'-Epsilon; PP2A B Subunit Isoform R5-Epsilon; PP2A, B Subunit, PR61 Epsilon; PP2A, B Subunit, B56 Epsilon; PP2A, B Subunit, B' Epsilon; PP2A, B Subunit, R5 Epsilon
Immunogen A synthesized peptide derived from human PPP2R5E
Additional Information
Gene ID 5529
Other Names Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform, PP2A B subunit isoform B'-epsilon, PP2A B subunit isoform B56-epsilon, PP2A B subunit isoform PR61-epsilon, PP2A B subunit isoform R5-epsilon, PPP2R5E
Protein Information
Name PPP2R5E
Function The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1661
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