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KD-Validated Anti-PRKAR2A Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PRKAR2A Rabbit Monoclonal Antibody AGI1668
    Western blotting analysis using anti-PRKAR2A antibody (Cat#AGI1668). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PRKAR2A antibody (Cat#AGI1668, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-PRKAR2A Rabbit Monoclonal Antibody AGI1668
    Western blotting analysis using anti-PRKAR2A antibody (Cat#AGI1668). PRKAR2A expression in wild-type (WT) and PRKAR2A shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PRKAR2A antibody (Cat#AGI1668, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-PRKAR2A Rabbit Monoclonal Antibody AGI1668
    Flow cytometric analysis of PRKAR2A expression in HAP-1 cells using anti-PRKAR2A antibody (Cat#AGI1668, 1:2,000). Green, isotype control; red, PRKAR2A.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P13861
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1265
Calculated MW Predicted, 46 kDa , observed , 52 kDa
Gene Name PRKAR2A
Aliases Protein Kinase CAMP-Dependent Type II Regulatory Subunit Alpha; PRKAR2; Protein Kinase, CAMP-Dependent, Regulatory Subunit Type II Alpha; CAMP-Dependent Protein Kinase Type II-Alpha Regulatory Subunit; PKR2; Protein Kinase, CAMP-Dependent, Regulatory, Type II, Alpha; CAMP-Dependent Protein Kinase Regulatory Subunit RII Alpha; Protein Kinase A, RII-Alpha Subunit
Immunogen A synthesized peptide derived from human PKA R2
Additional Information
Gene ID 5576
Other Names cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2A, PKR2, PRKAR2
Protein Information
Name PRKAR2A
Synonyms PKR2, PRKAR2
Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Cellular Location Cytoplasm. Cell membrane. Note=Colocalizes with PJA2 in the cytoplasm and the cell membrane
Tissue Location Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1668
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