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KD-Validated Anti-PDZ And LIM Domain 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PDZ And LIM Domain 1 Rabbit Monoclonal Antibody AGI1672
    Western blotting analysis using anti-PDLIM1 antibody (Cat#62538). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDLIM1 antibody (Cat#62538, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-PDZ And LIM Domain 1 Rabbit Monoclonal Antibody AGI1672
    Western blotting analysis using anti-PDLIM1 antibody (Cat#62538). PDLIM1 expression in wild type (WT) and PDLIM1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PDLIM1 antibody (Cat#62538, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-PDZ And LIM Domain 1 Rabbit Monoclonal Antibody AGI1672
    Flow cytometric analysis of PDLIM1 expression in HepG2 cells using anti-PDLIM1 antibody (Cat#62538, 1:2,000). Green, isotype control; red, PDLIM1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession O00151
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1310
Calculated MW Predicted, 36 kDa ; Observed, 36 kDa
Gene Name PDLIM1
Aliases PDLIM1; PDZ And LIM Domain 1; CLP36; CLP-36; HCLIM1; Elfin; CLIM1; Carboxyl Terminal LIM Domain Protein 1; PDZ And LIM Domain Protein 1; LIM Domain Protein CLP-36; Epididymis Secretory Protein Li 112; C-Terminal LIM Domain Protein 1; PDZ And LIM Domain 1 (Elfin); HEL-S-112
Immunogen A synthesized peptide derived from human PDLIM1
Additional Information
Gene ID 9124
Other Names PDZ and LIM domain protein 1, C-terminal LIM domain protein 1, Elfin, LIM domain protein CLP-36, PDLIM1, CLIM1, CLP36
Protein Information
Name PDLIM1
Synonyms CLIM1, CLP36
Function Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed:10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line Note=Associates with actin stress fibers
Tissue Location Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1672
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