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KD-Validated Anti-TAF15 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-TAF15 Rabbit Monoclonal Antibody AGI1701
    Western blotting analysis using anti-TAF15 antibody (Cat#AGI1701). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TAF15 antibody (Cat#AGI1701, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-TAF15 Rabbit Monoclonal Antibody AGI1701
    Western blotting analysis using anti-TAF15 antibody (Cat#AGI1701). TAF15 expression in wild type (WT) and TAF15 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TAF15 antibody (Cat#AGI1701, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-TAF15 Rabbit Monoclonal Antibody AGI1701
    Flow cytometric analysis of TAF15 expression in HepG2 cells using anti-TAF15 antibody (Cat#AGI1701, 1:2,000). Green, isotype control; red, TAF15.
    detail
  • ICC - KD-Validated Anti-TAF15 Rabbit Monoclonal Antibody AGI1701
    Immunocytochemical staining of HepG2 cells with anti-TAF15 antibody (Cat#AGI1701, 1:1,000). Nuclei were stained blue with DAPI; TAF15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q92804
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1830
Calculated MW Predicted, 62 kDa , observed , 62 kDa
Gene Name TAF15
Aliases TAF15; TATA-Box Binding Protein Associated Factor 15; RBP56; TAF2N; Npl3; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase II, N, 68kD (RNA-Binding Protein 56); TAF15 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 68kDa; TATA-Binding Protein-Associated Factor 2N; HTAFII68; TAFII68; TATA Box-Binding Protein-Associated Factor 2N (RNA-Binding Protein 56); 68 KDa TATA-Binding Protein-Associated Factor; TBP-Associated Factor 15; RNA-Binding Protein 56; RBP56/CSMF Fusion; TAF(II)68
Immunogen A synthesized peptide derived from human TAF15
Additional Information
Gene ID 8148
Other Names TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor, TAF(II)68, TAFII68, RNA-binding protein 56, TAF15, RBP56, TAF2N
Protein Information
Name TAF15
Synonyms RBP56, TAF2N
Function RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed:21256132). Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
Cellular Location Nucleus. Cytoplasm. Note=Shuttles from the nucleus to the cytoplasm
Tissue Location Ubiquitous. Observed in all fetal and adult tissues
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1701
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