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KD-Validated Anti-TOE1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-TOE1 Rabbit Monoclonal Antibody AGI1702
    Western blotting analysis using anti-TOE1 antibody (Cat#62623). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TOE1 antibody (Cat#62623, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-TOE1 Rabbit Monoclonal Antibody AGI1702
    Western blotting analysis using anti-TOE1 antibody (Cat#62623). TOE1 expression in wild type (WT) and TOE1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TOE1 antibody (Cat#62623, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-TOE1 Rabbit Monoclonal Antibody AGI1702
    Flow cytometric analysis of TOE1 expression in C2C12 cells using anti-TOE1 antibody (Cat#62623, 1:2,000). Green, isotype control; red, TOE1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q96GM8
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1860
Calculated MW Predicted, 57 kDa , observed , 60 kDa
Gene Name TOE1
Aliases TOE1; Target Of EGR1, Exonuclease; HCaf1z; TOE-1; Target Of EGR1, Member 1 (Nuclear); Target Of EGR1 Protein 1; PCH7
Immunogen A synthesized peptide derived from human TOE1
Additional Information
Gene ID 114034
Other Names Target of EGR1 protein 1, TOE1
Protein Information
Name TOE1
Function Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. Involved in the maturation of snRNAs and snRNA 3'-tail processing (PubMed:28092684).
Cellular Location Nucleus, nucleolus. Nucleus speckle. Note=Localizes to nuclear speckles
Tissue Location Widely expressed..
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1702
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