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KD-Validated Anti-VPS28 Subunit Of ESCRT-I Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-VPS28 Subunit Of ESCRT-I Rabbit Monoclonal Antibody AGI1715
    Western blotting analysis using anti-VPS28 antibody (Cat#AGI1715). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-VPS28 antibody (Cat#AGI1715, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-VPS28 Subunit Of ESCRT-I Rabbit Monoclonal Antibody AGI1715
    Western blotting analysis using anti-VPS28 antibody (Cat#AGI1715). VPS28 expression in wild type (WT) and VPS28 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-VPS28 antibody (Cat#AGI1715, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-VPS28 Subunit Of ESCRT-I Rabbit Monoclonal Antibody AGI1715
    Flow cytometric analysis of VPS28 expression in C2C12 cells using anti-VPS28 antibody (Cat#AGI1715, 1:2,000). Green, isotype control; red, VPS28.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q9UK41
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1755
Calculated MW Predicted, 25 kDa , observed , 25 kDa
Gene Name VPS28
Aliases VPS28; VPS28 Subunit Of ESCRT-I ; Vacuolar Protein Sorting-Associated Protein 28 Homolog; ESCRT-I Complex Subunit VPS28; VPS28, ESCRT-I Subunit; Vacuolar Protein Sorting 28 Homolog (S. Cerevisiae); Vacuolar Protein Sorting 28-Like Protein; Yeast Class E Protein Vps28p Homolog; Vacuolar Protein Sorting 28 (Yeast); Vacuolar Protein Sorting 28 Homolog; H-Vps28
Immunogen A synthesized peptide derived from human VPS28
Additional Information
Gene ID 51160
Other Names Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, VPS28
Protein Information
Name VPS28
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
Cellular Location Cell membrane. Late endosome membrane; Peripheral membrane protein
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1715
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