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KD-Validated Anti-Synaptosome Associated Protein 29 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Synaptosome Associated Protein 29 Rabbit Monoclonal Antibody AGI1718
    Western blotting analysis using anti-Synaptosome associated protein 29 antibody (Cat#AGI1718). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Synaptosome associated protein 29 antibody (Cat#AGI1718, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Synaptosome Associated Protein 29 Rabbit Monoclonal Antibody AGI1718
    Western blotting analysis using anti-Synaptosome associated protein 29 antibody (Cat#AGI1718). Synaptosome associated protein 29 expression in wild type (WT) and Synaptosome associated protein 29 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Synaptosome associated protein 29 antibody (Cat#AGI1718, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Synaptosome Associated Protein 29 Rabbit Monoclonal Antibody AGI1718
    Flow cytometric analysis of Synaptosome associated protein 29 expression in HepG2 cells using anti-Synaptosome associated protein 29 antibody (Cat#AGI1718, 1:2,000). Green, isotype control; red, Synaptosome associated protein 29.
    detail
  • ICC - KD-Validated Anti-Synaptosome Associated Protein 29 Rabbit Monoclonal Antibody AGI1718
    Immunocytochemical staining of HepG2 cells with anti-Synaptosome associated protein 29 antibody (Cat#AGI1718, 1:1,000). Nuclei were stained blue with DAPI; Synaptosome associated protein 29 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O95721
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1855
Calculated MW Predicted, 29 kDa , observed , 27 kDa
Gene Name SNAP29
Aliases SNAP29; Synaptosome Associated Protein 29; SNAP-29; Soluble 29 KDa NSF Attachment Protein; CEDNIK; Cerebral Dysgenesis, Neuropathy, Ichthyosis And Keratoderma Syndrome; Vesicle-Membrane Fusion Protein SNAP-29; Synaptosomal-Associated Protein, 29kDa; Synaptosomal-Associated Protein 29; Synaptosomal-Associated Protein, 29kD; Synaptosome Associated Protein 29kDa
Immunogen A synthesized peptide derived from human SNAP29
Additional Information
Gene ID 9342
Other Names Synaptosomal-associated protein 29 {ECO:0000312|HGNC:HGNC:11133}, SNAP-29 {ECO:0000312|HGNC:HGNC:11133}, Soluble 29 kDa NSF attachment protein {ECO:0000312|HGNC:HGNC:11133}, Vesicle-membrane fusion protein SNAP-29, SNAP29 (HGNC:11133)
Protein Information
Name SNAP29 (HGNC:11133)
Function SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also plays a role in ciliogenesis by regulating membrane fusions.
Cellular Location Cytoplasm. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q9Z2P6}; Peripheral membrane protein. Cytoplasmic vesicle, autophagosome membrane; Peripheral membrane protein. Cell projection, cilium membrane; Peripheral membrane protein. Note=Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic Localizes to the ciliary pocket from where the cilium protrudes
Tissue Location Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1718
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