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KD-Validated Anti-STAT5A Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-STAT5A Rabbit Monoclonal Antibody AGI1719
    Western blotting analysis using anti-STAT5A antibody (Cat#62641). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-STAT5A antibody (Cat#62641, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-STAT5A Rabbit Monoclonal Antibody AGI1719
    Western blotting analysis using anti-STAT5A antibody (Cat#62641). STAT5A expression in wild type (WT) and STAT5A shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-STAT5A antibody (Cat#62641, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-STAT5A Rabbit Monoclonal Antibody AGI1719
    Flow cytometric analysis of STAT5A expression in C2C12 cells using anti-STAT5A antibody (Cat#62641, 1:2,000). Green, isotype control; red, STAT5A.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P42229
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1920
Calculated MW Predicted, 91 kDa , observed , 92 kDa
Gene Name STAT5A
Aliases STAT5A; Signal Transducer And Activator Of Transcription 5A; STAT5; MGF; Epididymis Secretory Sperm Binding Protein
Immunogen A synthesized peptide derived from human STAT5a
Additional Information
Gene ID 6776
Other Names Signal transducer and activator of transcription 5A, STAT5A, STAT5
Protein Information
Name STAT5A
Synonyms STAT5
Function Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL- induced transcription. Regulates the expression of milk proteins during lactation.
Cellular Location Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1719
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